FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9474085
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCG2406.735572E-515.66714494
TCGCGTA1259.544936E-515.0401490-91
AGAGTGT319150.09.95549694
AAGAGTG376000.09.61279294
AAAGAGT398300.09.44040994
GACGCGT4150.00666157959.0624799
GAGTGTA93200.08.82537694
GAAAGAG513100.08.76639594
TAGGGAA644550.08.7578594
GTAGGGA716050.08.64477294
GCCGCGA5558.6516794E-48.4723494
GTGTAGG851200.08.27163394
TGTAGGG779450.08.24914594
CGTGTAG920850.08.24828294
GGAAAGA568200.08.23062794
GGGAAAG621700.08.18763894
GCGTAAA2900.00134425548.10369592-93
TATCACG5900.00158391657.9700052
TCGTGTA984900.07.921857494
AGGGAAA665900.07.88415794
Produced by FastQC (version 0.11.5)