FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4487540
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG702.1853084E-433.57326594
CGCGAGT1151.3775675E-424.5230894
TCGTACG550.00306195921.36266146-47
TACGATC1805.6751887E-620.8870073
CGATCGG1059.543801E-720.1426178-79
GTCGCGA1101.4917969E-619.22553884-85
ACGTTCG2304.687222E-516.3452599
TCGCGAG1307.3408773E-616.26776384-85
CGCTAGC2104.3458366E-415.6649054
CGATAGG2457.80199E-515.3994341
ACGATCG950.00403078714.84192578-79
AACGCGT1452.0635343E-514.58594868-69
CGATCGT1452.0640773E-514.58554152-53
TACGCCG1452.0642945E-514.58537954-55
ACGTTAT3555.885322E-714.5617425
AAACGCG1158.6522574E-414.30592766-67
TATCTCG25900.014.15521494
CGCCGAT2051.9851905E-713.75623650-51
ACGCGTG2752.0843602E-413.7194961
TAGTCGT4554.836329E-813.42930694
Produced by FastQC (version 0.11.5)