FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4487540
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA1452.5396406E-522.6847494
CGTACGA550.00306331321.36103262-63
CCGATCG800.00128094317.62206666-67
CGAACGT1203.430694E-617.62118370-71
TTTAGCG3402.0063453E-816.5824248
CGACCGC2658.815901E-615.9566738
TATACCG2658.815901E-615.9566738
GTCGCCG2151.4006218E-915.30216558-59
TTCGCGG1850.003378727515.2406215
CTCGCGA1158.6397375E-414.30866422-23
AACGCGA1158.6682127E-414.30244478-79
ACGTTCG2000.00567198814.0974179
CTACGTC3709.3784547E-713.9730621
CGCGAGA2409.762516E-812.72740280-81
CCGTACG1350.002899512412.183762-63
GTACGCT1400.003802967311.75000942-43
TCGTTCG2054.107275E-511.46565950-51
CCGATTG2502.1930664E-611.28082644-45
GCGATCC4601.0742007E-511.2376984
CGTACGG2105.2526608E-511.19235532-33
Produced by FastQC (version 0.11.5)