FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14193838
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA137550.09.12306194
ACACGTC179450.09.088160594
ACGTCTG156400.08.9851394
ATCGTAC5851.5033677E-48.8384422
GCACACG233400.08.15537894
CACACGT212100.08.13233794
CACGTCT172050.07.867336894
CGGCGAT9801.7227958E-67.6730869
GATCGGA518900.07.49957494
CGTATCG4453.0839365E-57.393266790-91
AGAGCAC350500.07.32140894
TAGATCG217550.07.258888794
ATCGGAA478300.07.2026794
CTCGCTA11251.7551338E-67.10750151
TATCGAT8005.490047E-47.049871494
AGCACAC318000.07.02031394
CGGAAGA448200.06.983808594
ATACGAT11452.315759E-66.98335271
TCGGAAG469250.06.98101394
GAGATCG259900.06.94408394
Produced by FastQC (version 0.11.5)