FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14193838
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG1504.4804308E-412.53258282-83
GTCGTGT164150.09.44890894
CGTGTAG149400.09.34360494
GCGTCGT205700.09.07120194
AGCGTCG231500.09.05508394
CGTCGTG186350.09.05469194
TCGTGTA147250.08.9533392-93
GAGCGTC268300.08.58388794
AGAGCGT301200.08.37968894
GTGTAGG159200.08.17796594
AAGAGCG330350.08.10978194
ATATGCG14356.020855E-107.53764341
GATCGGA519100.07.497006494
GTAGGGA146450.07.221072794
ATCGGAA478550.07.1599394
TAGATCG224200.07.106776794
TCGGAAG471400.07.009294594
AGATCGG555600.06.94527694
GTTACGT9507.2286566E-56.9304931
CGGAAGA454050.06.914827394
Produced by FastQC (version 0.11.5)