FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859961
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA400500.08.17948694
ACACGTC514750.08.13536694
ACGTCTG440800.08.08208594
CACACGT582250.08.02366394
CACGTCT480500.07.60995394
CTCGTAT14951.8189894E-107.54511494
AGTCACT173150.07.51887894
TCTGAAC412850.07.308672494
GCACACG630000.07.281234794
CTGAACT375450.07.11036794
GTCTGAA458950.07.096817594
TGAACTC363850.07.091624394
CAGTCAC204250.07.04133794
AATCTCG24700.07.04045294
GAACTCC344350.06.71522894
AACTCCA321600.06.707989794
GAGCACA810350.06.640925494
AGAGCAC836000.06.61145194
TCCAGTC282850.06.53028594
AGCACAC733150.06.365775692-93
Produced by FastQC (version 0.11.5)