FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859961
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT2500.001816658412.9665954
GTCGTGT496350.08.24855394
GTAGGGA355850.08.17346594
CGTGTAG430750.08.14999794
CGTCGTG547900.08.140264594
TGTAGGG387900.08.11900294
AGAGTGT187400.08.03206794
GTGTAGG411150.07.95276194
TAGGGAA327950.07.88025294
TCGTGTA451150.07.73014494
GCGTCGT593950.07.43115994
AAGAGTG240800.07.19330894
GAGCGTC711050.07.19088794
AAAGAGT241650.07.168005594
GAGTGTA58250.07.15583294
AGCGTCG645700.06.968563692-93
AGAGCGT772850.06.89753194
AAGAGCG840550.06.76625594
AGGGAAA413750.06.3020894
ATCGGAA1155800.06.18296594
Produced by FastQC (version 0.11.5)