FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8365109
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC9550.013.78042494
GATCGCA21900.013.52086194
CGCTCGA3004.478965E-412.5328748
TCGCGAT3451.0533569E-412.261525
AGATCGC25900.010.34386994
TCGCAAT15800.09.81668394
ATCGCAA21850.09.4647594
CGTCTGA217800.08.86935194
TACGGCC8151.028191E-68.649998
ACGTCTG239300.08.56350894
ACACGTC279100.08.36959594
ATACGGC6859.14687E-58.2332747
CACACGT310350.08.14776494
GCACACG341700.07.84039294
CACGTCT259000.07.64981992-93
GCGTAGT6959.5961656E-47.43924335
TCCGATA6350.00327349837.4018793
TCTGAAC234250.07.06270394
AGCACAC413800.06.894552794
TAGCGTA3750.00215293026.8930672-73
Produced by FastQC (version 0.11.5)