FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7410714
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT2051.986864E-713.75545172-73
TCGCGAC1402.501878E-413.42739670-71
TCTCGCG2903.363099E-412.9656955
ATTCGCG1300.002181779412.65453320-21
CGCGTCG2052.9628718E-612.614192-93
CGTTCGA3451.0543766E-412.2602119
CGTCGAA3156.7303027E-411.94078294
ACGCGAC2105.2433963E-511.19440790-91
AACGCGA2452.1549002E-510.55333988-89
CGGTCGC2901.1264074E-610.53818390-91
TACGCGT2757.9516444E-410.25427157
ATACGCC3750.002883988710.03025794
AACGCGC1950.0040029629.64067116-17
CTAACGG3900.00400311979.6405089
TCGGTAC4400.00100014299.613128
CGCTCGA2000.00493647259.39879838-39
TCGTGTA230600.09.37889994
TAGCGCC4550.00135793239.2968315
CGTGTAG219600.09.27062194
GCGAACC2300.00149863169.1970224-25
Produced by FastQC (version 0.11.5)