FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4892636
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT23200.010.73475594
AGTCACG31800.010.19591494
ACGACTA18000.010.18113894
GTCACGA29050.09.867063594
CGACTAG17400.09.58701492-93
TCACGAC27050.09.554316594
ACTAGTA18550.09.372642594
GACTAGT19800.08.78093494
ACGCAAT2500.00319519768.46120366-67
CACACGT168150.08.35561894
GCACACG190400.08.07021394
ATACGCT5250.00497118158.0563113
CACGTCT140950.07.901098794
ACGTCTG129850.07.85275494
ACCGAGT6000.00187964097.83252532
CGTCTGA117750.07.761808492-93
CTGAACT116900.07.67755794
GAGCACA248950.07.43683794
TACGAAT7854.4230238E-47.1839722
ACACGTC150950.07.128638394
Produced by FastQC (version 0.11.5)