FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4892636
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGC3700.002590342210.1608662
TAGGGAA100800.08.86071494
CGGTGCG4350.0098258258.6424889
CGTCGTG161100.08.52045894
GCGTCGT176850.08.37394
GAGCGTC215950.08.18484294
TCGTGTA136250.08.03887194
GTAGGGA110450.08.00143394
AGCGTCG194800.07.987570394
GCGTTAG2650.00541199987.98068546-47
TGTAGGG118150.07.95741394
CGTGTAG127100.07.7669394
AGAGTGT54550.07.75574394
AAAGAGT78900.07.50704894
GTGTAGG123950.07.4333694
GTCGTGT144600.07.282078794
AAGAGCG258450.07.275437494
AAGAGTG71250.07.191463594
AGAGCGT235050.07.139759594
GACGAGC6800.0063825186.91088342
Produced by FastQC (version 0.11.5)