FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14188032
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT54400.013.82055194
TCACGCG82200.013.20518394
GCGAGTA50350.012.69239894
CACGCGA72950.011.8521494
CGAGTAA46000.011.03240494
ACGCGAG64450.011.00926494
GTCACGC109000.010.64816494
AGTCACG128900.09.66044694
ACGTCTG482850.07.980055394
CGTCTGA440150.07.953528494
CACACGT622500.07.85806594
TCTGAAC460100.07.63930194
CTGAACT419800.07.633895494
ACACGTC561500.07.632193694
CACGTCT525100.07.472203794
GCACACG679650.07.321749794
TGAACTC392750.07.07091494
AGCACAC802700.07.03062694
GTCTGAA514700.06.938469494
GAACTCC362100.06.799970694
Produced by FastQC (version 0.11.5)