FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7968520
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATT25500.09.76643594
ACCGGAT28800.09.6263194
CACCGGA31800.09.45699694
CGTCTGA167550.09.2548694
GTCACCG40950.08.95037294
ACACGTC218200.08.42019894
ACGTCTG186200.08.4035994
CACGTCT205800.08.31105994
CACACGT247450.08.22244294
GCACACG272000.08.01584794
CGGATTG22900.08.0025794
AGAGCAC380200.07.58851294
TCACCGG40600.07.46507292-93
CTGAACT174650.07.26432994
AGTCACC74750.07.16628494
AAGAGCA452150.07.160395694
AGCACAC332450.07.123682594
GTCTGAA212300.07.10485894
TCTGAAC188650.07.0490494
GAACTCC154150.07.01107694
Produced by FastQC (version 0.11.5)