FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7968520
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG181000.08.72727894
TGTAGGG165100.08.6850294
GTCGTGT210050.08.66176694
GTGTAGG178400.08.64364994
TCGTGTA189300.08.61781194
GTAGGGA152350.08.5786894
CGTCGTG233400.08.50021294
TAGGGAA143000.08.41631694
GCGTCGT256250.08.3843794
AGCGTCG280400.08.24907494
AAGAGCG379750.08.19555494
AGAGCGT343950.08.11912694
GAGCGTC312600.07.47455894
AGAGTGT82300.07.42611494
AAAGAGT116600.07.217263794
CGGAAGA516050.06.69597394
TCGGAAG548450.06.66899994
GATCGGA596200.06.60800294
AGATCGG637150.06.603884794
GAAGAGC474400.06.550506694
Produced by FastQC (version 0.11.5)