FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15028873
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA447900.08.60275494
AATCTCG28900.08.45493494
ACGTCTG492200.07.98122294
ACACGTC578250.07.923074294
CACGTCT537350.07.80906394
CACACGT639050.07.63250794
CTCGTAT21150.07.553935594
GCACACG713100.07.426398394
TCTGAAC460300.07.299108594
TGAACTC409300.07.232751494
GTCTGAA528600.07.209388794
AGAGCAC951300.06.930186394
GAACTCC374900.06.89369494
GAGCACA911450.06.887765494
AGCACAC835900.06.77387994
GTCACAC170350.06.758163594
ATCGGAA1346600.06.494003394
CTGAACT431900.06.440850792-93
AGTCACA230800.06.270752494
GAAGAGC1141800.06.247215394
Produced by FastQC (version 0.11.5)