FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11241418
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGGA64250.011.62904594
ACCGGAT51850.011.14747294
GTCACCG81150.010.30745794
TCACCGG78800.09.66070694
CCGGATT53200.09.62797494
CGTCTGA339500.08.73392994
CACGTCT408700.08.28992794
ACGTCTG372050.08.23505294
CGGATTG53250.08.2069894
ACACGTC432050.07.972417494
TCTGAAC345450.07.86253694
CACACGT481950.07.71248994
CTGAACT316850.07.504415594
TGAACTC294200.07.347425594
GCACACG534100.07.32016494
GAACTCC274750.07.01239294
AGCACAC631300.06.930011394
AGAGCAC710450.06.832613594
GAGCACA690250.06.787483794
AGTCACC135800.06.74768694
Produced by FastQC (version 0.11.5)