FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12953667
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18000.08.74761644-45
TATGCCG19900.08.73927248-49
TCGTATG19950.08.59938544-45
CGTCTGA440600.08.59809294
CGTATGC21450.07.7790746-47
ACACGTC560250.07.709836594
CGCGAAA3100.0025931297.580327566-67
ACGTCTG481800.07.55067494
ATGCCGT23900.07.47329848-49
CACGTCT527300.07.460693494
TCTGAAC444200.07.152858794
TCCAGTC283800.06.98894494
GCACACG674000.06.952592494
CTGAACT407350.06.85378194
CACACGT618650.06.82248994
AGTCACA219900.06.818313694
AACTCCA349950.06.80947294
TGAACTC405500.06.583684494
GTCTGAA483900.06.536886794
CTCCAGT306150.06.52478494
Produced by FastQC (version 0.11.5)