FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11601875
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAG43150.011.00125594
ACGACTA49450.010.45509694
CACGACT59500.010.42698294
GTCACGA76550.09.20974794
TCACGAC67200.09.022494
GACTAGT50950.08.85581894
AGTCACG89050.08.76144694
CACGTCT332600.07.941740594
ACACGTC352550.07.93227794
CACACGT394750.07.858206394
CGTCTGA277350.07.795272494
ACGTCTG305800.07.34669694
ACTAGTA46350.07.09822894
GCACACG429200.07.06320294
AATCTCG21150.06.666724794
GAACTCC263350.06.603437494
GTCTGAA351950.06.51689194
TCTGAAC318300.06.452773694
TCGTATG15301.2369674E-76.451036594
AGAGCAC569800.06.277169794
Produced by FastQC (version 0.11.5)