FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6933996
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC2702.5867848E-819.1573684
CGTAACG1052.148326E-517.90149764-65
TAACGCA2402.5465852E-1115.66527832-33
CGCGAAT1401.4814086E-515.1066286-87
GCGTCGA3203.190893E-614.6951033
CGTACGT1654.418511E-614.24121448-49
TACGCTC5351.4188117E-1014.05533594
GCGTAAT4755.640686E-913.8631031
AGTCGCG3103.8487473E-513.6522243
CGCTCGA3801.2613145E-613.6106346
CTACGCT3508.072262E-613.4347465
CGCATAA2550.001875402812.9117131
TACGCCG2409.7545126E-812.7283642-43
CGATCGT1300.00218405112.65277148-49
CGTCCGA2052.9772382E-612.60863932-33
TAACGGT5403.414425E-812.1892049
AATCGTA4659.101768E-712.1346085
CGCTTAA5051.8693754E-712.1081561
GTCGTAC2003.2012016E-511.74895932-33
CGTCGTA1801.5690754E-411.74853554-55
Produced by FastQC (version 0.11.5)