FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12532172
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG295150.08.585649594
TCGTGTA307200.08.58556494
GCGTCGT410000.08.39371894
GTCGTGT337150.08.18544194
CGTCGTG371150.08.10695394
TGTAGGG271700.08.080894
AGCGTCG446550.07.990957394
GTAGGGA255200.07.97690494
GTGTAGG288250.07.77993994
TAGGGAA237600.07.776304294
AGAGTGT141900.07.388386294
AGAGCGT544700.07.23292294
GAGCGTC494650.07.204408694
AAGAGCG592900.07.128618294
CGGAAGA763000.06.59921294
ATCGGAA829800.06.464565894
AAAGAGT194350.06.362068794
TAGATCG330100.06.138452594
AAGAGTG177750.06.136284494
AGATCGG939100.05.96248294
Produced by FastQC (version 0.11.5)