FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5349945
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22250.016.89905594
TCTCGTA35950.014.51197494
ATCTCGT44700.013.56390194
CGTATGC19650.013.39454394
AATCTCG49600.012.60295694
CTCGTAT26600.012.19294192-93
TATGCCG14650.012.19125594
GCGAAAT75800.012.09115794
GTGCGAA100300.011.3869694
GGTGCGA121500.010.59928994
CGGTGCG130500.010.58861894
TGCGAAA89200.010.48553794
GCCGTCT12750.010.32167794
ACGGTGC152700.010.21883794
GCGAAAA5103.356029E-510.13736294
CGAAATC70900.09.87739694
GTCACGG208900.09.67458794
ATGCCGT16100.09.63364294
TCACGGT182750.09.02718294
AGTCACG225100.08.89480794
Produced by FastQC (version 0.11.5)