FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12615973
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT5253.1269155E-711.637985
GTCACGT72200.010.15562794
AGTCACG88500.09.87845594
TCACGTG68000.09.60782894
CACGTGT68350.09.48986294
GTCGACG6202.8436782E-59.0963238
ACTCGCG3956.568516E-78.923573522-23
CGTCTGA312150.08.65812594
ACGTCTG348500.08.26755494
ACACGTC403450.08.23662194
CGCTAGT3153.349639E-48.20753792-93
CACGTCT379150.07.971118594
GCGATCG3651.9159005E-47.72561362-63
CACACGT449300.07.720391394
AGCGTAC3050.00221098527.704505416-17
AATCTCG24850.07.56576794
ACGTGTT58650.07.37291494
GCACACG491900.07.1568994
AGCACAC590300.07.10250394
CTGAACT315350.07.049976394
Produced by FastQC (version 0.11.5)