FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6262850
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA175700.08.29276594
ACGTCTG193300.08.29147894
CACGTCT210650.08.14405694
ACACGTC226100.07.940945694
TCTGAAC185600.07.44524194
TCGTATG8351.1235115E-47.317559294
CTGAACT172500.07.165987594
CAGTCAC92150.07.03871194
CACACGT250100.07.028575494
GCACACG269700.06.88375794
TCCAGTC118300.06.754194794
TGAACTC167800.06.750477394
AACTCCA144800.06.556804794
GTCTGAA208050.06.551483694
CTCCAGT127100.06.434474594
AGCACAC320700.06.31666394
AAGATCG104200.06.314946794
CTCGTAT8206.864866E-86.30522292-93
AGTGGTC38500.06.226139594
ACTCCAG148100.06.156814694
Produced by FastQC (version 0.11.5)