FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6262850
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG188200.08.54328294
TCGTGTA198950.08.53063794
GTAGGGA159700.08.3310594
GTGTAGG181000.07.94805994
GTCGTGT217850.07.85528794
TAGGGAA149850.07.78060194
TGTAGGG169650.07.64845194
AAGAGTG107250.07.53961894
AGAGTGT84900.07.25407294
CGTCGTG237100.07.237349594
GCGTCGT257150.07.148393694
AGCGTCG278500.07.123697894
GAGTGTA29000.06.808783594
GAGCGTC305000.06.782207594
AAAGAGT113700.06.73977494
AAGAGCG358600.06.541969394
AGAGCGT330700.06.51102794
ATCGGAA496200.06.01637994
AGGGAAA197450.06.00014494
CGGAAGA458050.05.983756594
Produced by FastQC (version 0.11.5)