FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6044783
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC1902.0239607E-417.3165894
TATCTCG54200.016.39004794
TCGTATG29300.015.88127194
CGTAACG1350.002897770413.9252958
ATGCCGT17600.013.88699794
TATGCCG18250.013.64993994
CGTATGC27850.013.501513594
TCTCGTA45800.012.62284794
CTCGTAT33950.012.46006894
GTATCTC72600.012.36557894
CCGTTAT3551.374917E-411.9161365
TTCGCGG2800.003801208511.7506345
ATCTCGT50850.011.46168394
CGTACCG2100.007360540311.1899697
ATGCGCG4255.9373833E-511.0583238
GTACGCC2156.687049E-510.93000836-37
TCGCACG3050.00718907310.7874675
CAAGTAT117050.010.68139494
AAGTATC102350.010.65412594
TCGCGGA3100.00811184110.6133886
Produced by FastQC (version 0.11.5)