FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6044783
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT3103.869221E-513.6444644
GCGCGAC3207.706727E-411.7495949
ATCGTTC5203.5155335E-610.8455993
TAGATCT34300.010.82953694
GGTAACG3050.007169971710.79133294
CTAGCGG5608.516725E-610.0709141
GCGTCGG11700.09.64594
GTGTAGA74000.09.53095594
GAGTGTA110650.09.0936794
AGAGTGT385350.08.85845894
AAGAGTG437250.08.67801994
CGTTAAT4900.00266560948.6322122
AAAGAGT475200.08.60834694
GATCTCG24200.08.54897894
CTAACGC2550.00382777478.293694520-21
ATCTCGG27950.08.24311194
GAGTGTC87800.08.14002994
CGCGAGT2900.00134492238.10323428-29
AGCGCGA5350.0058867547.90620338
TAGCGGG5950.00173025177.89875652
Produced by FastQC (version 0.11.5)