FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16343096
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG44700.08.72616394
TCGTATG25900.07.80227894
CGTCTGA676600.07.59867894
CTCGTAT28550.07.57189394
ACACGTC849650.07.395100694
CGTATGC25500.07.371783394
ACGTCTG739950.07.360948694
TGAACTC609850.07.3438394
TCCAGTC402150.07.245299394
CTCCAGT444400.07.233271694
CACACGT937700.07.171804494
ATCTCGT55150.07.15791494
CTGAACT622050.07.018481394
AACTCCA510550.06.9772494
TCTGAAC676050.06.909717694
CACGTCT799250.06.89124694
AGTCACA302250.06.88795394
GCACACG1014850.06.876656594
CCAGTCA402850.06.77774994
GTCTGAA757550.06.69985294
Produced by FastQC (version 0.11.5)