FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8172588
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG753.2983713E-521.93026222-23
TCGTACG1001.4087407E-518.80087578-79
CGTCTTA6950.018.2610173
CGCGTAA1650.001571244217.0928044
CGCGATT900.002814027215.66514486-87
CGACCTA4401.9426807E-914.9524517
CGTAGCG1301.332467E-414.46221278-79
CGTACGA2050.00666774613.7576222
TCGCGAA1553.8714672E-513.64391938-39
TTAGGCG3456.9991474E-613.62169694
TAGTGCG2450.001394381413.4300593
CGCGCTA2102.6639827E-713.42833474-75
ACCGCGA2802.5027763E-413.4266917
ATCCGCG4809.73032E-812.73057652
TAACGGG3751.6399132E-512.5411691
GTACGCT3004.4828522E-412.5315789
CGGCGTA2652.6409907E-812.41624224-25
GGGTCGA9250.012.19325994
GCGCAAT2700.002883294112.1928021
AATCCGA14500.011.9944322
Produced by FastQC (version 0.11.5)