FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8172588
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG551.0199542E-425.63336686-87
CGCGAAT1452.7885108E-919.44885672-73
GCGTAAA1902.0261615E-417.3142416
GTACGAT4401.1277734E-1016.022572
TAGTCGC4051.0486474E-815.0852565
CGCGATT1000.00567000114.09847914-15
TACGATC4153.406407E-612.4577083
GTACGAC4153.406407E-612.4577083
ATCCGCG4203.900781E-612.307678
AGCGTTA4305.0654016E-612.0231373
TCGACCG4751.1855482E-611.8719689
CTACGCT5152.43861E-711.8630166
TCGCGAA1607.710913E-411.74905716-17
CGTACCG3051.5032128E-811.55567290-91
CGTTCGA3309.984184E-411.3922949
CGTAAAT6654.436515E-911.3071217
AACTACG3752.2903625E-411.2798883
CCTACGC7101.029548E-911.2526525
GTTAGCG4255.9358335E-511.0587142
CGACGTT6101.8573519E-710.78717494
Produced by FastQC (version 0.11.5)