FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11305991
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT58600.011.71044494
TCGTATG26800.011.39978694
CTCGTAT33100.011.36004894
AATCTCG54350.010.98303592-93
ACTAATC89650.010.38085494
TCTCGTA51800.010.16263894
TATGCCG20750.09.51369894
GTCACTA141750.08.98595894
CAGTCAC498900.08.67689494
CGTATGC23900.08.65312592-93
TAATCTC102050.08.47467394
AGTCACA337100.08.31008994
CACTAAT126350.08.146894
AGTCACT190050.08.13666194
TCACTAA157650.07.751710494
CTAATCT105250.07.725776794
TCACAGT308400.07.528886394
TCCAGTC458400.07.280009394
CTCCAGT481450.07.21458694
ACTCCAG534750.07.13712794
Produced by FastQC (version 0.11.5)