FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9324419
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG89300.09.73688694
TCACCTA115700.09.09943794
CTAAGGT76950.09.03967894
CACCTAA98000.08.96840894
TCGTATG11901.8189894E-128.68911694
TAAGGTC65950.08.62323994
GTCACCT148800.08.21240394
CAGTCAC188850.08.1631394
AGTCACC158600.07.971663594
CGTCTGA414050.07.821071694
TCTGAAC407300.07.77759594
ACGTCTG453300.07.765973694
CTCGTAT16550.07.66770994
AACTCCA304400.07.65837994
CACGTCT494650.07.56336294
CTGAACT373700.07.55877194
CCTAAGG85350.07.43412994
ACACGTC529050.07.391395694
GAACTCC328550.07.267115694
TGAACTC357250.07.19639494
Produced by FastQC (version 0.11.5)