FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6410204
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG1300.00804503918.0760637
CTCGTAT13450.012.92905594
CGTCGTA54900.012.07074694
CACGTCG75350.011.66395194
GTCGTAG52400.011.39094594
AATCTCG23250.011.32015594
AGTCACG114050.010.87917694
ACGTCGT65050.010.62080494
CGTAGAA43050.010.58975694
TAGTACG2452.1606504E-510.55076566-67
CGTATGC11750.010.39974894
CGACCGC4155.8455084E-410.1922869
TCACGTC91000.010.17447994
GTCACGT95550.09.985105594
TCGTATG12200.09.63091594
TCGTAGA52250.09.53469894
TACGCGG4050.00545305679.2857534
GTTAGCG2300.00150075399.19557328-29
ATCGGAC4300.0089156658.7458856
ATCTCGT22750.08.67671394
Produced by FastQC (version 0.11.5)