FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539517
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG155200.010.96322394
ACCGGAT92050.010.92726294
CGGATTG93750.010.17761994
CCGGATT90100.09.54657894
CACCGGA120000.09.51801694
ACGTCTG656850.09.11642694
TCACCGG158350.09.08290594
CGTCTGA601800.08.27894794
CTCGTAT15900.08.27717894
CACGTCT722450.08.13899794
CACACGT862650.08.09120694
GTCTGAA640250.08.04604394
TGAACTC480850.08.03495794
TCTGAAC579800.07.98508394
CTCCAGT350400.07.98131294
CTGAACT526800.07.976514394
GGATTGC79150.07.95747594
AACTCCA410600.07.93296794
AGTCACC222100.07.76674294
GAACTCC455650.07.74693694
Produced by FastQC (version 0.11.5)