FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854269
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG2800.003783141311.7581991
TCGTATG12400.010.23390594
TATCTCG19850.08.99751194
CGTATGC12050.08.19089994
TTACGCA6807.600585E-47.60369256
CGTCTGA294400.07.439562394
GTCACAC106100.07.35346494
AGTCACA144700.07.210805494
TCTGAAC296250.07.10753494
CTCCAGT202550.07.10049394
CTCGTAT14900.07.09745292-93
CTGAACT270750.07.082572594
ATCTCGT26200.06.996206394
ACACGTC363000.06.99175994
CAGTCAC149500.06.979287694
CACACGT397850.06.9463694
ACGTCTG317300.06.917451494
GCACACG434300.06.88282394
TGAACTC262550.06.87414294
CACGTCT341400.06.828376394
Produced by FastQC (version 0.11.5)