FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14885952
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG47150.010.16774494
CTCGTAT31150.09.05314294
GTCACTA222600.08.59360494
CACTATC192850.08.5544894
CGTATGC26550.08.49733694
TCTCGTA49300.08.29427594
CTATCAG172150.08.27259494
TCACTAT219350.08.22810394
TATCAGC166950.07.910902594
ACTATCA198900.07.727145794
CGTCTGA708900.07.59148394
AGTCACT295200.07.562813394
TCTGAAC688700.07.370548294
ACGTCTG774500.07.245844494
TCGTATG26900.07.16383892-93
TCCAGTC428450.07.163399794
CAGTCAC340650.07.13326394
ATCTCGT54200.07.110836594
CACGTCT837750.07.063458494
ACTCCAG529850.07.034388594
Produced by FastQC (version 0.11.5)