FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14885952
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT323900.07.965839494
GTAGGGA635200.07.79830694
AAGAGTG388600.07.607080594
TAGGGAA579150.07.6035994
GAGTGTA105450.07.531998694
CGTGTAG766750.07.379770894
TGTAGGG681950.07.3257394
GTGTAGG729950.07.288254394
TCGTGTA802000.07.266369394
AAAGAGT409250.07.21175794
AGCGTCG1091200.06.787690694
GAAAGAG553500.06.75873894
GTCGTGT867300.06.740951594
CGTCGTG938050.06.59325894
GCGTCGT1007550.06.51628394
GGGAAAG626800.06.42572394
GAGCGTC1188050.06.376765794
TTCGCGG9757.4423477E-46.26898536
GGAAAGA601250.06.26105694
AGAGCGT1278900.06.24348394
Produced by FastQC (version 0.11.5)