FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4744224
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA3650.00231391610.299926
TCGTGTA112300.09.54467794
GTCGTGT124100.09.05382894
GTGTAGG103400.08.72944894
CGTGTAG105800.08.620296594
TAGGGAA85700.08.61241694
CGTCGTG138150.08.50736994
GTAGGGA93000.08.088037594
GCGTCGT151550.07.94127494
AGCGTCG165050.07.77594694
TGTAGGG98600.07.581556392-93
AGAGCGT200300.07.440197594
AGAGTGT53400.07.21902894
AAGAGCG219150.07.207819594
GAGCGTC182850.06.87807792-93
CGGAAGA284900.06.732460594
GAAGAGC265800.06.703326794
TAGATCG126450.06.617703494
CAATTCG4350.00142410476.48273456-57
ATCGGAA311150.06.37600794
Produced by FastQC (version 0.11.5)