FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11662204
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA72900.010.8964594
ACCCGTG106650.010.57733494
TCACCCG133850.010.53485994
CACCCGT116650.010.07351694
CCCGTGA114550.09.84786294
CTCGTAT19700.09.78235694
AATCTCG30750.09.47703394
GTCACCC176900.09.24649794
ATCTCGT34800.09.184594
CCGTGAG97550.08.96213594
CGTCTGA555800.08.05935494
AGTCACC207550.08.03954494
AACTCCA398850.07.978199594
TCCAGTC322350.07.84477494
ACACGTC707700.07.63790994
CTCCAGT345200.07.55697694
ACGTCTG608500.07.54674794
CACGTCT658500.07.53047794
CAGTCAC262300.07.50829994
TCGTATG18850.07.480588494
Produced by FastQC (version 0.11.5)