FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11662204
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGTG291250.08.65585294
AGAGTGT241500.08.49866194
AAAGAGT305150.08.24621194
GAGTGTA79000.07.94824494
TAGGGAA445550.07.9404394
GTGTAGG564450.07.811917394
TGTAGGG519350.07.795556594
GTCGTGT685400.07.766543494
CGTGTAG592400.07.704375394
GTAGGGA482600.07.660959794
TCGTGTA633700.07.57198594
GGAAAGA448200.07.32891594
CGTCGTG744350.07.1640594
GCGTCGT804950.06.97399294
GAGCGTC945550.06.70016894
GGGAAAG471500.06.62884294
AGCGTCG868300.06.605491694
TACGTAG8600.00129632196.53690248
AGGGAAA509250.06.46871294
GAAAGAG436200.06.17697394
Produced by FastQC (version 0.11.5)