FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12932216
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC516000.08.48743394
CGTCTGA401500.08.414988594
CACACGT574600.08.2679194
ACGTCTG442900.07.851203494
AATCTCG27600.07.83175294
TCTGAAC410450.07.647620794
CACGTCT480400.07.629694
GCACACG640200.07.44273394
CTGAACT377750.07.401550394
AGTCACT173250.07.3503294
AACTCCA311350.07.30477294
GAACTCC356450.06.960581394
AGCACAC755650.06.902596594
GTCTGAA467600.06.83352294
TCGTATG15950.06.77606194
TCCAGTC246400.06.732001394
AGAGCAC848150.06.68720794
CAGTCAC211600.06.684377794
GAGCACA812200.06.653421494
TGAACTC365750.06.642267794
Produced by FastQC (version 0.11.5)