FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8756973
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT1351.8373577E-413.92547154-55
TAGTGCG2500.001626887813.1579924
CGTAACG1801.0826459E-513.05792470-71
ACGCGAG3651.2521421E-512.8745995
CGCGAGT2652.6502676E-812.41316434-35
GCGTACG1852.0228997E-411.43192852-53
TTTTACG5203.5204012E-610.8444998
CGTAGCG2000.0049217799.40221674-75
CCGTATG5701.1356415E-49.0693091
CTATACG4700.00182149479.00201994
ATGCGCG4200.00737369068.95101454
ACCGATT5901.652083E-48.7618741
AGAGTGT255050.08.53396694
GAGTGTA80600.08.51555794
GAACGCG5558.671633E-48.47036894
GTACGAT2500.0032048958.45844316-17
AAAGAGT325600.08.44630494
GAGTGTT76950.08.4307494
GTGTAGG611200.08.14531494
AAGAGTG306850.08.04319294
Produced by FastQC (version 0.11.5)