FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636162
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG1054.9112714E-1126.85631264-65
TCGCGAA650.00804399718.07667772-73
CGGATAT4809.7310476E-812.7305415
AATCTCG24650.012.39355794
CGAGCGT2951.1726115E-711.15271384-85
GTCACGT101600.010.87110494
AGTCACG119150.010.25601894
CGTCTGT73450.09.7903794
TCACGTC95950.09.52750492-93
ACGTCTG522600.08.78667694
CACGTCT574700.08.45626994
CAGTCAC184300.08.23714494
CGCAATT5200.0045244328.1411981
CGTCTGA421450.08.10751694
TCGCTAG7553.214329E-58.0924187
TGAACTC348800.07.97709494
CTCCAGT263400.07.94042594
CGGGAGT9651.3621393E-67.7935876
CCGGTCG5500.00754219347.69062667
GAACTCC322600.07.6488294
Produced by FastQC (version 0.11.5)