FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636162
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG900.002805421115.67234912-13
CGTAACG1553.8737395E-513.64307756-57
CGCGTAT2055.2339316E-410.31555680-81
CGGTTCG3800.00321312279.9002763
CGACCGT2851.0527703E-59.89325350-51
CGGTTAG3305.9047507E-69.2567584-85
TACCGAT5751.2407906E-48.9958175
AGAGTGT176550.08.704658594
AAAGAGT235350.08.66657194
TGTAGGG396100.08.48345894
CGTGTAG457800.08.24349794
ATTATCG5300.0053696947.98763751
TAGGGAA335650.07.939042694
GTGTAGG428000.07.917047594
AACGATT7151.4963189E-47.89207125
TCGTGTA484700.07.853870494
CGACGAC6605.5055605E-47.8364478
ACGCTCG3000.00188029447.83249936-37
GTAGGGA362300.07.796108294
CGTCGTG579600.07.61393194
Produced by FastQC (version 0.11.5)