FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7225067
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG651.0896099E-525.30575820-21
CGTACGC1351.4601625E-524.3674968
CGCTAGT1103.1998326E-517.09019382-83
TCGCGAC1551.2644159E-716.6752210-11
CGACCGC4602.2191671E-1015.3248086
TCGCCGG3203.210671E-614.6863775
GCGTACG1158.65271E-414.30592654-55
CGACGAA2350.00101907713.9996082
TGCGATA4554.8432412E-813.4281943
TAGCACG3951.959579E-613.0876065
TTCGCGT1901.889821E-512.36927252-53
ATCGTAC3105.901081E-412.1290281
CGCGAAA1450.004943382511.34443178-79
GCGTTGA5002.195542E-611.2796832
CTCGCGA3752.2928866E-411.2785139
ATGCGCG3350.001131297411.223414
GATTGCG2950.00561642911.1516116
GCGATAG3000.00636633110.9653738
CGCGATA1953.2950635E-410.84577578-79
ACGCGTC2208.45389E-510.68063222-23
Produced by FastQC (version 0.11.5)