FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9634073
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG154300.09.74782794
GTCGTGT175950.09.34980394
AGCGTCG236400.09.0665394
TCGTGTA158400.08.78335294
GCGTCGT212750.08.24067994
CGTCGTG195350.08.18067694
GTGTAGG153400.08.1197894
TAGGGAA136900.07.93107894
GAGCGTC265750.07.76452694
GTAGGGA142600.07.44925194
AGAGCGT292400.07.314047394
TAGATCG221100.07.29170594
TGTAGGG148150.07.24929692-93
ATCGGAA460750.06.81451394
AAGAGCG322000.06.714685494
CAGATCG137800.06.65133994
GATCGGA495150.06.63535494
GTTCGTA8550.00117126556.595726
AGATCGG527800.06.57219994
GAAGAGC419200.06.447187494
Produced by FastQC (version 0.11.5)