FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5954246
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCG1303.1616108E-421.6924936
CTCGTAT14600.013.84049194
AATCTCG23450.011.222280594
TCGTATG11500.011.03320994
CGTATGC10701.2732926E-119.66217494
GTCACAT145850.09.31166594
CAGTCAC190800.09.30999494
TCACATC138100.09.08559794
CCGTACG2100.00736376138.95253342-43
TAATCTC44550.08.86068994
AGTCACA176300.08.63631894
ATGCGGC6555.41611E-58.6107626
ATCTCGT27450.08.55979994
AACTCCA308300.08.55116694
GAACTCC342550.08.42326394
TGAACTC370100.08.39302194
CCAGTCA226750.08.372786594
TCTCGTA24300.08.31568794
CGTCTGA476150.08.06332694
GCGCTTA5250.00497551968.0555889
Produced by FastQC (version 0.11.5)