FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5954246
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC1909.059075E-619.7875825
AGAGTGT188850.09.58253494
GAGTGTA55450.09.49408394
AAAGAGT244050.08.47442994
AAGAGTG221450.08.44780694
GTAGGGA404700.08.36249494
TAGGGAA372950.08.34341794
TGTAGGG439200.08.122992594
GTGTAGG481400.07.94794694
GGGAAAG362550.07.92154594
AGGGAAA395950.07.799407594
CGCTTAT3356.5083825E-47.714967312-13
CGTGTAG514400.07.63909594
GGAAAGA346750.07.55049394
TCGTGTA546850.07.460846494
GCGTCGG12058.863208E-87.4114594
GTCGTGT593450.07.326460494
GAAAGAG311200.07.29531694
GAGTGTG77700.07.259334694
GAGTGTT57900.07.143987794
Produced by FastQC (version 0.11.5)