FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474988
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT93200.07.968099694
GCACACG102150.07.82211494
ACACGTC83350.07.78192694
CGTCTGA66300.07.65677292-93
TATCGCC3100.00259202377.58048470-71
CACGTCT79650.07.376287594
GAACTCC63800.07.29336894
GAATCCG6500.00410767.2314485
CTGAACT72600.07.121470594
AGAGCAC141800.07.060294
AGCACAC126350.06.956326594
TCGGAAG193650.06.86882794
CGGAAGA184100.06.867712594
ACGTCTG72800.06.843999492-93
TCTGAAC77900.06.6972994
GAGCACA139900.06.584942394
ATCGGAA198950.06.485357392-93
GTCACAC29050.06.310039594
GTCTGAA88900.06.29156794
AACTCCA67000.06.243510294
Produced by FastQC (version 0.11.5)