FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474988
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA58850.08.70763494
GCGTCGT95500.08.46730494
AGCGTCG104650.08.35591194
CGTCGTG86500.08.15258494
TCGTGTA71450.07.96165394
GTCGTGT77950.07.961189794
GAGCGTC117500.07.76218594
GTAGGGA63400.07.63779794
AGAGTGT34500.07.631133694
TGTAGGG69250.07.60358594
AGAGCGT130100.07.552471694
CGTGTAG69350.07.185045792-93
GTGTAGG70800.07.071091792-93
TACGGAG3700.00186644686.98684576-77
CGGAAGA183200.06.903144494
TCGGAAG194600.06.885288794
AAGAGCG140950.06.803544592-93
GAGTGTA13301.0013537E-86.71617694
AAGATCG56700.06.55034594
GTGCACG7900.00371344766.54407459
Produced by FastQC (version 0.11.5)