FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978676
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT804.783459E-429.374798
GCGTTAG4301.4006218E-915.3018322
CGAACCG1106.1832415E-414.95462678-79
ACCGTCG4809.7435986E-812.7290766
CGATCGG1300.002182606112.65386176-77
TTGTACG2700.002897369412.1847931
TCGATCG2155.064856E-612.02337160-61
GTATCGT3601.5674396E-411.7496212
CGTTAGA7652.3646862E-1111.6728273
TAGTCGC3400.00128026511.0584682
CTTCGCG1850.002588221810.1620926-27
ATACCGC3254.450976E-710.12300524-25
GCGAGTA3501.1333577E-710.07148378-79
CGATAAA6102.159899E-610.0163226
CTTAACG2503.0658132E-49.40048386-87
CTTACGA6052.1290352E-59.3220141
GTAGGGA291050.08.89987994
CTACCGA5851.5046418E-48.8375448
GTCGGTT4350.0098178738.64341
ACTCGCG2450.00266453678.63273660-61
Produced by FastQC (version 0.11.5)